Figure 3

The magnitude of the APα-induced intracellular calcium response changes at different DIV. (A) Bar graph indicates the magnitude of the [Ca²⁺]_i increases, high response (defined as >65 nM [Ca²⁺]_i rise over the baseline) or low response (defined as <45 nM [Ca²⁺]_i rise over baseline), in [Ca²⁺]_i over the baseline in hippocampal neuron cultures at different DIV treated with 500 nM APα. (B) Bar graph indicates the percentage of hippocampal neurons that respond to 500 nM APα with a high or a low response at different DIV. Data are mean ± SEM (N = 3 independent experiments with at least 36 neurons/condition/experiment). *p < 0.01, **p < 0.001 versus DIV 0.