Block of chloride-mediated inhibition inducesseizure-like activity in medullary slice preparations. Three sequential sample recordings of integrated preBötC activity from a single medullary slice preparation. A. Activity recorded in recorded in drug free aCSF. B. Gabazine (20 μM) and Strychnine (1 μM) induce seizure-like bursting (brackets) characterized by increased burst frequency and elevated baseline, while slightly decreasing the frequency of population bursts generated between seizure-like bursts. C. Antagonism of A1R with NCPA (1 μM) eliminated seizures for this slice and decreased population burst frequency.