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Table 5 qRT-PCR verification of selected transcripts in ExpG.

From: Bioinformatic and statistical analysis of the optic nerve head in a primate model of ocular hypertension

Transcript

577 FX

578 FX

566 FX

529 FX

APOE

qRT-PCR

1.11 ± 0.13

1.15 ± 0.16

3.55 ± 0.38

3.07 ± 0.38

 

Microarray

1.75 ± 0.24

-1.15 ± 0.15

2.95 ± 0.52

1.24 ± 0.16

BMP2

qRT-PCR

1.92 ± 0.13

-1.29 ± 0.19

3.30 ± 0.16

3.25 ± 0.35

 

Microarray

1.34 ± 0.06

-1.30 ± 0.12

2.19 ± 0.36

2.14 ± 0.56

CAPG

qRT-PCR

1.26 ± 0.09

1.72 ± 0.25

5.70 ± 0.35

1.66 ± 0.16

 

Microarray

1.54 ± 0.20

1.05 ± 0.09

3.93 ± 0.36

-1.13 ± 0.15

GAP43

qRT-PCR

2.49 ± 0.15

1.39 ± 0.24

3.32 ± 0.33

3.18 ± 0.50

 

Microarray

1.52 ± 0.07

-1.04 ± 0.04

2.83 ± 0.26

1.55 ± 0.21

GPNMB

qRT-PCR

2.40 ± 0.15

3.17 ± 0.31

90.68 ± 9.07

3.77 ± 0.27

 

Microarray

1.68 ± 0.26

1.27 ± 0.28

29.64 ± 6.44

2.04 ± 0.50

NEFH

qRT-PCR

1.74 ± 0.24

4.86 ± 0.35

1.65 ± 0.14

56.48 ± 6.53

 

Microarray

2.95 ± 0.21

6.18 ± 0.85

2.53 ± 0.26

42.68 ± 4.88

NEFL

qRT-PCR

2.46 ± 0.14

7.75 ± 0.37

-1.63 ± 0.13

5.38 ± 0.97

 

Microarray

2.22 ± 0.14

3.59 ± 0.21

1.15 ± 0.06

1.71 ± 0.29

STMN2

qRT-PCR

2.44 ± 0.10

13.12 ± 0.95

-1.04 ± 0.07

4.03 ± 0.33

 

Microarray

1.69 ± 0.11

3.46 ± 0.32

-1.04 ± 0.06

1.77 ± 0.36

  1. qRT-PCR verification in four paired glaucomatous macaques of eight selected transcripts. Fold change (i.e. fold increase or fold decrease; 'FX') was calculated within each paired sample as described in Methods. Standard error of fold change was calculated as described in Methods.
  2. Numerical values for microarray are from human definitions, with the exception of GPNMB, which was not detected by human definitions.