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Figure 2 | BMC Neuroscience

Figure 2

From: Bioinformatic and statistical analysis of the optic nerve head in a primate model of ocular hypertension

Figure 2

Axonal morphology of monkey optic nerves with experimental glaucoma in sagittal sections of the ONH (Macaque #2; Supplemental Table 3). (A) Sagittal view of the normal optic nerve head stained for neurofilament heavy (red, NF200). Superimposed in blue is the DIC image of the structure of the lamina cribrosa (LC). (B) Similar view of the contralateral ExpG eye. Note irregular size and morphology of the axons (arrows) in the optic nerve head in experimental glaucoma (A and B, scale bar, 50 μm). NB = nerve bundles. (C) Co-localization of GAP43 (green) and phosphorylated NEFH (red) in bulb-like structures (Ax) in ExpG. Astrocytes (arrows) in the pial septa (PS) show abundant GAP43 staining. NB = nerve bundle (scale bar, 25 μm). (D) Double immunostaining for stathmin (red) and phosphorylated neurofilament (green) shows oligodendrocyte processes (arrows) on axonal bulb-like structures (Ax) in ExpG. PS = pial septa (scale bar, 25 μm). (E) Co-localization of protein disulfide isomerase (PDI), a marker of endoplasmic reticulum, and phosphorylated NEFH (SMI31) in some axons in the nerve bundles (NB) in normal post laminar optic nerve suggested the presence of ribosomes, seen as green granules within axons. Notice that glial cells in the pial septa (PS) (arrowheads) stain positive for PDI (scale bar, 40 μm) (F). PDI and phosphorylated NEFH co-localization in axons in nerve bundles (NB) in ExpG (arrows). Abnormal axon (Ax) stained with NEFH and PDI in ExpG optic nerve (scale bar, 25 μm). Nuclei are stained with DAPI.

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