Distribution of sNPF-Gal4 driven GFP expression in adult brain. The Gal4 line NP6301 drives GFP in neurons that largely correspond to the sNPFp-IR neurons in the adult brain. A. Overview of brain in confocal section at level of mushroom body lobes. Strong expression is seen in axons in the α, β and γ-lobes and heel (h) of the mushroom body, and in processes in neuropil of the subesophageal ganglion (SEG). In glomeruli of the antennal lobe (AL) labeling is weaker at this level. Several groups of neuronal cell bodies are seen (e. g. PI and DLN2). A neuropil at the base of the lobula (arrow) is strongly expressing sNPF-Gal4 (see Fig. 10 for details). B. Detail of mushroom body lobes. The α, β and γ-lobes and heel (h) contain fibers expressing sNPF, whereas the α' (not seen) and β' do not. The arrow indicates a set of small median neurons (DMN in E). C. Overview of dorsal brain at level of mushroom body calyces (Ca). Kenyon cells (KC) and their processes express sNPF. A pair of large median neurons (MN1, see Fig. 7A) can be seen. D. Detail of mushroom body calyces (Ca) with numerous Kenyon cells (KC) and sets of DNL1 neurons. E. A set of dorsal median neuron cell bodies (DMN) located between the tips of the α-lobes. F. Cell bodies of the median neurons in the pars intercerebralis (PI) and their axons (arrow), dorsal to the central body (CB). Two layers of sNPF-expresing processes are seen in the central body. Strong sNPF expression is seen in axons of the cross-sectioned mushroom body peduncles (P).