I n situ hybridization with nucleotide probe to snpf in the adult brain. The brain and subesophageal ganglion (SEG) is shown in frontal views (dorsal is up). A. Schematic tracing of snpf expressing cell bodies in brain. Red cell bodies are posterior, black ones anterior and yellow are posterior and large intensely labeled ones. The cell body groups in the protocerebrum have been designated as follows: DLN1 and 2, dorso-lateral neurons; PI, pars intercerebralis neurons; DMN, dorsal median neurons, M1 – 4, median neurons. OL1 and 2, optic lobe neurons; NC, possible neurosecretory cells; In subesophageal ganglion: S1 – 3, subesophageal neurons. These designations are used in the following figures for adult neurons. Note that the mushroom body Kenyon cells and numerous small cell bodies distal to the medulla are not shown here. B. Micrograph of posterior portion of the brain with in situ signal in Kenyon cells (KC), large, possibly neurosecretory cells, and cell bodies in the subesophageal ganglion (SEG). The framed area is shown in D. Some of the cell bodies from A are indicated. C. Detail of cluster of dorsal lateral neurons (DLN1) posterior to the Kenyon cell group (left hemisphere). DMN, small median neurons. D. Dorsal brain (right hemisphere) with Kenyon cells (KC), a putative neurosecretory cell (NC), clusters of small cells (DMN and M3) and a pair of median cells (M1).