Figure 13

Distribution of sNPF in relation to putative cholinergic neurons in the adult brain. Brain is shown in frontal sections (dorsal is up). Antiserum to sNPFp (magenta) and Cha-Gal4-driven GFP (Green) revealed some neurons where markers are colocalized (note that the P{Cha-GAL4.W}19B is a fusion with cytoplasmic GFP). A1 – 3. Distribution of sNPF in axon terminations of olfactory receptor neurons (ORNs) in a subset of antennal glomeruli (AG). The Cha expression is seen in both ORNs and projection neurons of the antennal lobe. B1 – 3. sNPF immunolabeling in cell bodies of a subset of Cha expressing ORNs in the antennae. The boxed area in B1 is shown magnified in B1' and B3'. The arrows in B1' and B3' indicate one of the ORNs expressing both markers C1 – 3. Cha and sNPF is also colocalized in interneurons in the deutocerebrum and subesophageal ganglion (arrows and boxed areas). Note that the GFP is mainly nuclear here. These images are slightly tilted; dorsal (d) is in direction of arrow. The boxed areas are shown enlarged in D and D'. E and F. Colocalization of Cha-expression and sNPF immunolabeling in a set of small cells laterally in the subesophageal ganglion (S3 neurons in Fig. 7A). G1 – 2. In the central body (fan-shaped body shown here) there is no neuronal colocalization of Cha and sNPF markers. The sNPFp antiserum labels neuronal processes in two layers (UD and LD) of the fan-shaped body, whereas Cha-expression is seen other processes in the UD and in a layer between this and the LD. H. There is no sNPFp-immunolabeling in the ellipsoid body (EB) of the central complex, whereas Cha expression is seen. VAL, ventral accessory lobe; BL, beta-lobe of mushroom body; AL, antennal lobe. The magnification of H is the same as in A.