Distribution of sNPF in relation to putative cholinergic neurons in the larval CNS. Antiserum to sNPFp (magenta) and Cha-Gal4-driven GFP (green) were used as markers. Single confocal sections are shown (anterior is upwards). A1 – 3. In the brain (protocerebrum) there are a few cell bodies in a dorso-lateral cluster displaying colocalized markers: one (arrowed in A2) is seen in boxed area of A3. This is also shown in enlarged view in A2' and A3'. B1 – 2. More dorsally in the brain a few other cell bodies in the protocerebrum display colocalized markers (arrows). C1 – 3. In the ventral nerve cord (abdominal ganglia) colocalized markers were seen in a small number of cell bodies (arrows). The boxed area (shown in higher magnification in C2' and C3') contains a pair of dorsal neurons (DP) in A1 that colocalize Cha and sNPFp. A few more posterior abdominal neurons with colocalized markers were found in adjacent sections.