Figure 1

Cell bodies of snpf /sNPF expressing neurons in the larval CNS. In situ hybridization reveals snpf transcript in A-D and immunocytochemistry displays cell bodies in E. A. In situ signal in neurons of the protocerebrum (Pro) of the brain and subesophageal ganglion (SEG). DLN, dorsolateral neurons (including possible neurosecretory cells). B. Higher magnification of the right brain hemisphere in another specimen reveals numerous Kenyon cells (KC) of the mushroom body and three of the strongly labeled cell bodies dorsally (asterisk) that may represent neurosecretory cells. C and D. In the ventral nerve cord segmentally arranged cell bodies display in situ signal. Eight abdominal neuromeres are indicated (A1 – A8). The boxed areas are shown in higher magnification in C', C" and D': in C" and D' lateral cell body clusters are shown with 4 – 5 neurons in each hemineuromere and in C' the two dorsal cell bodies (DP) of A1 are visible. E. Tracing of cell bodies labeled with antiserum to sNPF precursor-derived peptide (sNPFp). In this tracing only strongly labeled cell bodies are depicted and the Kenyon cells were ommitted. Additional small cell bodies with variable labeling intensity can be seen in brain and ventral nerve cord. Red cell bodies are dorsal, black ones ventral or lateral and yellow ones dorsal and with larger diameters (yellow cellbodies in the brain probably correspond to the ones indicated with asterisk in B). Two groups of cell bodies are indicated, DLN and DP. T1 – T3, thoracic neuromeres, other abbreviations as in A – D.