Figure 3
From: Expression of ATF3 and axonal outgrowth are impaired after delayed nerve repair
I mages of double labeling of ATF3 and neurofilament (a, b) and NCAM and neurofilaments (c-f). Double immunochemical staining of ATF3 and neurofilaments (a, b) from the distal nerve after immediate repair (day 0, a) and delayed repair at day 30 (b). Note ATF3 stained Schwann cells close to regenerating fibers. Double immunochemical staining of NCAM (green) and neurofilaments (red) at the contralateral uninjured side (c), at the injured side where sciatic nerve was repaired immediately (d), repaired with delay of 30 days (e) and 90 days (f) after injury. Note the difference in staining of neurofilament and NCAM between c, d, e and f. Scale bar = 50 μm.