A and B: Bar graph demonstrating the fluorescence intensity for 5-HT1B, AT1, ETB and ETA receptors in the middle cerebral artery and micro-vessels. There was a significant increase of 5-HT1B, AT1, and ETB protein expression in MCAO as compared to control. This was prevented with U0126 treatment starting at 0 h and 6 h in the MCA and in the micro-vessels but not 12 h. Results are presented as the mean percentage relative to control ± s.e.m.; n = 5. *P < 0.05, **P < 0.01. Statistically significant differences between the groups are shown; §< 0.05, §§ < 0.01 significant difference between MCAO and treated groups.