A and B: Bar graph demonstrating the fluorescence intensity for pERK1/2, and pElk-1 in the MCA and micro-vessels. There was a significant increase of pERK1/2, and pElk-1 protein expression in MCAO as compared to control. This was prevented with U0126 treatment starting at 0 h and 6 h but not 12 h. Results are presented as the mean percentage relative to control ± s.e.m.; n = 5. *P < 0.05 significant difference between control groups and MCAO, § < 0.05 significant difference between MCAO and treated groups.