Figure 4

MMP-28 antibodies specifically inhibit MMP-28. A) Polyclonal antibodies to MMP-28 inhibit proteolytic activity. 10 nM MMP-28 was pre-incubated for 1 hour at room temperature with 5 or 30 nM anti-MMP-28 pAb180 or pAb183 and subsequently incubated with the Omni-MMP substrate for 24 hours. Data shown as relative fluorescent units or tabulated as % inhibition compared to no antibody controls. Summary of two independent experiments ± SEM (* p ≤ 0.05, ** p ≤ 0.01). B) 100 nM MMP-28, 100 nM MMP-10 or, 10 nM MMP-2 was pre-incubated for one hour at room temperature with pAb180 or pAb183 or IgG control at the indicated concentrations and subsequently incubated with the Omni-MMP substrate. Relative fluorescence was measured at the indicated time points. Summary of two independent experiments ± SEM.