Entorhinal cortex microglial and astrocytic staining patterns evolve on a similar timescale as observed in the 3xTg-AD hippocampus. Coronal mouse brain sections (30 μm) were prepared from 3xTg-AD mice sacrificed at 2 (A, I), 3 (B, J), 6 (C, K), 9 (D, L), 12 (E, M), 15 (F, N), 18 (G, O), and 26 months of age (H, P) and were processed for immunohistochemistry to detect activated microglia using an anti-F4/80 specific monoclonal antibody (A–H) and astrocytes using an anti-GFAP specific monoclonal antibody (I–P). Entorhinal cortex was examined for patterns of immunopositivity and photomicrographs were obtained at 10×. The insets in panels H and P represent 40× digitally magnified images of designated photomicrographs for better visualization of immunopositive cells. Scale bar in D represents 200 μm.