Anti-inflammatory effects of edaravone. Upper column: Iba-1 staining revealed that edaravone administration (250 mg/kg) at 30 minutes after 6-OHDA lesion significantly suppressed the number of Iba-1-positive cells (b), compared to rats without edaravone-administration (a), thus indicating anti-inflammatory effects of edaravone. Scale bar: 60 μm. Lower column: The graph demonstrates that 250 mg/kg of edaravone significantly suppressed the microglial proliferation, compared to rats without edaravone-administration. Data are shown as the cell number of Iba-1-positive cells +S.E. *p < 0.05 vs. rats without edaravone administration.