The percentage of motile mitochondria in wildtype and CK-B knockout neurons was determined. The first (red) frame and the 100th frame (green) of a movie were merged. Yellow mitochondria are non-motile mitochondria. Panel A) 1st and 100th frames of a movie of mitochondria in a wildtype neuron after merging. Panel B) displays the CK-B knockout equivalent. For wildtype (n = 12) and CK-B knockout (n = 15) movies were analyzed using the stack difference method (see methods). The percentage of motile mitochondria is presented in the scatter dot plot in panel C (p < 0.05). The percentage of motile YFP-APP particles in wildtype and CK-B knockout neurons was determined by analyzing 2 sets of merged frames from every movie (see also Additional Files 1 and 2). Panel D shows the average percentages (WT: 74%; CK-B: 76%; p = 0.57) of motile YFP-APP vesicles (error bars represent SEM). n = 22 for WT and n = 14 for CK-B(-/-).