Mitochondria of 7-day-old CK-B deficient and wildtype neurons were stained with rhodamine 123. Live imaging was applied to track these mitochondria. Average velocities were determined for mitochondria of wildtype cells (164 mitochondria) and CK-B deficient cells (138 mitochondria). The bar diagrams in panel A, display average velocities with error bars representing the standard deviation (SD). Panel B shows the distribution of mitochondrial velocities. Maximum velocities for individual tracked mitochondria were also determined and the average of these are presented in panel C. Error bars represent SD. D) The distribution of maximal velocities of individual mitochondria are shown for wildtype and CK-B knockout neurons.