Western blot analysis of p53 expression in Hcy-treated and untreated Neuro2a cells. After a 4 h Hcy treatment, in the presence or absence of SN50, the specific NF-κB inhibitor, cell proteins were probed with monoclonal antibodies against p53 and β-actin (figure top). A densitometric analysis of immunoblots (figure bottom) was also carried out after normalization against β-actin. Results were similar in three separate experiments. *p < 0.05 significant difference in comparison with untreated cells.