Figure 3

Effects of Hcy treatment on Bcl-2 and Bax expression in Neuro2a cells. Neuro2a cells were treated with 250 μm Hcy for 4 h in the presence or absence of SN50, the specific NF-κB inhibitor, that was added to cell cultures 20 min prior to incubation with Hcy. The immunoblots, with relative densitometric analysis after normalization against β-actin, show Hcy-evoked effects on the expression of anti-apoptotic and pro-apoptotic markers, such as Bcl-2 and Bax, respectively. Results are expressed as mean ± SEM of five independent experiments. *p < 0.05 compared with control cells, ^§p < 0.05 in comparison with Hcy-treated cells.