Effects of Hcy on ROS production (A) and cell viability (B) in neuroblastoma Neuro2a cells. ROS production was measured by DCF formation as reported by Lee et al. . Cell viability was evaluated by MTT assay as described in Materials and Methods. Results shown are mean ± SEM of three-five separate experiments. *p < 0.05 and **p < 0.01 significant differences in comparison with untreated cells; §p < 0.05 and §§p < 0.01 significant differences in comparison with Hcy-treated cells.