Figure 4

Low power views of a dorsal to ventral series of vibratome sections triple labeled for synapsin immunoreactivity (SYN; red), RFamide-like immunoreactivity (RF; green), and the nuclear marker (NUC; blue); conventional fluorescence combined with the Apotome structured illumination technique for optical sectioning. Note that section E is from another animal and inserted here because it provides a better view of the central body (CB). Section E is not perfectly horizontal so that the medial foramina of the olfactory lobes are not visible here. The arrows in C to F point to the cerebral artery that pierces the brain. Numbers 6, 9, 10 identify cell clusters. Letters A to F identify the non-columnar olfactory neuropils. Other abbreviations: AcN accessory lobe/neuropil, AMPN anterior medial protocerebral neuropil, AnN antenna 2 neuropil, LAN lateral antenna 1 neuropil, mF median foramen, OGT olfactory globular tract, ON olfactory lobe/neuropil, PC protocerebrum, pF posterior foramen, PMPN posterior medial protocerebral neuropil, xON side olfactory lobe/neuropil, VC ventral neuropil column.