Skip to main content
Figure 13 | BMC Neuroscience

Figure 13

From: Brain architecture in the terrestrial hermit crab Coenobita clypeatus(Anomura, Coenobitidae), a crustacean with a good aerial sense of smell

Figure 13

Details of the accessory lobe (AcN); confocal laser scan microscopy. A: Double labeling for synapsin immunoreactivity (SYN; green) and actin (ACT; red). The accessory lobe is located close to the exit point of the olfactory globular tract (OGT) from the olfactory lobe. Other abbreviations: F patch F of the non-columnar olfactory neuropil, LAN lateral antenna 1 neuropil, OGTNa accessory olfactory globular tract neuropil. B: Histochemical localization of actin (ACT) shows two fiber bundles (arrows) that extend from the accessory lobe towards the olfactory globular tract (OGT) but seem to pass underneath it. Color coded three dimensional visualization of a confocal stack, use red-green glasses to view. C: Color coded three dimensional visualization of a confocal stack (use red-green glasses to view) of a synapsin labeled specimen shows that the accessory lobes is composed of an array of evenly spaced spherical glomeruli. D: a surface reconstruction of four glomeruli obtained from a z-series of confocal images (synapsin immunoreactivity) that were directly loaded into Amira and processed for semiautomatic segmentation using Amira's "wrap" module. E: single optical section of the glomeruli (synapsin labeling) to show the regular structure of the synaptic neuropil within the glomeruli. F: Double labeling for serotonin immunoreactivity (5HT; green) and glutamine synthetase-like immunoreactivity (GS; red). A single glutamine synthetase positive cell (arrow) just adjacent to the lateral antenna 1 neuropil (LAN) extends branches towards and penetrates into several glomeruli of the accessory lobe.

Back to article page