Table 1 Summary table of the observed phenotypes and conductance properties of the N.at-Kv3.2 single and double S4 mutants

Wild-type

8

Inward rectifier

H325A

5

Delayed rectifier

V₅₀ = 51.9 ± 3.4 b = 21.8 ± 1.6

H325C

5

Delayed rectifier

V₅₀ = 62.5 ± 5.7 b = 22.9 ± 2.1

H325S

4

Delayed rectifier

V₅₀ = 60.6 ± 4.5 b = 19.2 ± 1.9

H325R

7

Delayed rectifier

V₅₀ = 69.9 ± 8.8 b = 27.3 ± 2.7

H325E

18

Inward rectifier

H325V

12

Inward rectifier

G331P

6

Inward rectifier

G331A

14

Inward rectifier

G331R

7

Delayed rectifier

V₅₀ = 73.8 ± 9.7 b = 23.8 ± 2.8

G331K

12

Delayed rectifier

V₅₀ = 36.0 ± 1.0 b = 15.2 ± 0.8

H325A + G331P

8

Inward rectifier

H325C + G331P

6

Inward rectifier

H325S + G331P

6

Inward rectifier

H325R + G331P

11

Inward rectifier

H325E + G331P

6

Inward rectifier

H325V + G331P

8

Inward rectifier

H325A + G331A

9

Inward rectifier

H325C + G331A

7

Inward rectifier

H325S + G331A

7

Inward rectifier

H325R + G331A

8

Inward rectifier

H325E + G331A

8

Inward rectifier

H325V + G331A

9

Inward rectifier

H325A + G331R

8

Inward rectifier

H325C + G331R

7

Inward rectifier

H325S + G331R

n/a

No detectable current

H325R + G331R

7

Inward rectifier

H325E + G331R

10

Inward rectifier

H325V + G331R

10

Inward rectifier

H325A + G331K

12

Combined

V₅₀ = 22.5 ± 1.8 b = 15.7 ± 1.5

H325C + G331K

7

Combined

V₅₀ = 15.6 ± 3.4 b = 23.7 ± 2.8

H325S + G331K

6

Combined

V₅₀ = 7.6 ± 0.4 b = 15.0 ± 0.4

H325R + G331K

6

Delayed rectifier

V₅₀ = 25.6 ± 1.2 b = 16.5 ± 1.0

H325E + G331K

7

Combined

V₅₀ = -27.5 ± 2.1 b = 23.3 ± 2.1

H325V + G331K

7

Combined

V₅₀ = 12.1 ± 1.2 b = 10.2 ± 1.1