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Table 1 Summary table of the observed phenotypes and conductance properties of the N.at-Kv3.2 single and double S4 mutants

From: A naturally occurring omega current in a Kv3 family potassium channel from a platyhelminth

Channel n Phenotype Delayed Rectifier Properties*
Wild-type 8 Inward rectifier  
H325A 5 Delayed rectifier V50 = 51.9 ± 3.4 b = 21.8 ± 1.6
H325C 5 Delayed rectifier V50 = 62.5 ± 5.7 b = 22.9 ± 2.1
H325S 4 Delayed rectifier V50 = 60.6 ± 4.5 b = 19.2 ± 1.9
H325R 7 Delayed rectifier V50 = 69.9 ± 8.8 b = 27.3 ± 2.7
H325E 18 Inward rectifier  
H325V 12 Inward rectifier  
G331P 6 Inward rectifier  
G331A 14 Inward rectifier  
G331R 7 Delayed rectifier V50 = 73.8 ± 9.7 b = 23.8 ± 2.8
G331K 12 Delayed rectifier V50 = 36.0 ± 1.0 b = 15.2 ± 0.8
H325A + G331P 8 Inward rectifier  
H325C + G331P 6 Inward rectifier  
H325S + G331P 6 Inward rectifier  
H325R + G331P 11 Inward rectifier  
H325E + G331P 6 Inward rectifier  
H325V + G331P 8 Inward rectifier  
H325A + G331A 9 Inward rectifier  
H325C + G331A 7 Inward rectifier  
H325S + G331A 7 Inward rectifier  
H325R + G331A 8 Inward rectifier  
H325E + G331A 8 Inward rectifier  
H325V + G331A 9 Inward rectifier  
H325A + G331R 8 Inward rectifier  
H325C + G331R 7 Inward rectifier  
H325S + G331R n/a No detectable current  
H325R + G331R 7 Inward rectifier  
H325E + G331R 10 Inward rectifier  
H325V + G331R 10 Inward rectifier  
H325A + G331K 12 Combined V50 = 22.5 ± 1.8 b = 15.7 ± 1.5
H325C + G331K 7 Combined V50 = 15.6 ± 3.4 b = 23.7 ± 2.8
H325S + G331K 6 Combined V50 = 7.6 ± 0.4 b = 15.0 ± 0.4
H325R + G331K 6 Delayed rectifier V50 = 25.6 ± 1.2 b = 16.5 ± 1.0
H325E + G331K 7 Combined V50 = -27.5 ± 2.1 b = 23.3 ± 2.1
H325V + G331K 7 Combined V50 = 12.1 ± 1.2 b = 10.2 ± 1.1
  1. Channels were expressed in X. laevis oocytes and their electrophysiological properties were evaluated. V50 and Boltzmann slope factor were obtained by fitting to the delayed rectifier components of the current-voltage data.