Distinctive sequence characteristics of N.at-K
3.2 from Notoplana atomata. Amino acid sequence alignment of the S2, S3, the S4 voltage sensor and the canonical pore of N.at-K
3.2 with other voltage-gated ion channels (Shaker – Drosophila Shaker B; Nt Kv3.2 – Notoplana atomata Kv3.2 channel; Nt Kv3.1 – Notoplana atomata Kv3.1 channel;RatKv1.2 – Rattus norvegicus Kv1.2 channel;RatKv1.2 – Rattus norvegicus Kv1.2 channel; RatNav1.2II – Rattus norvegicus Type 1.2 sodium channel, homology domain II; RatNav1.4II – Rattus norvegicus Type 1.4 sodium channel, homology domain II). The highly conserved acidic residues in S2 and S3 are in open rectangles, while the characteristic positive arginine/lysine repeats (R1–R6) in the voltage sensor are shaded in black. N.at-K
3.2 has a histidine (H325) at position R1 (blue) and a glycine (G331) at position R3 (red). The characteristic potassium selective GYG sequence is highlighted in grey in the alignment of the canonical pore.