Figure 5

Characterization of migration in lattice cultures. Distribution of mean cell speeds during 30 min observations in A) E15 and B) E17 cultures. C) Inhibition of myosin-II with 100 μM blebbistatin significantly reduced migration speed compared to DMSO control. D) Mean migration speed was the same in the second 30 min of transmitted light illumination compared to the first 30 min. A minimum of 35 cells from 3 separate cultures were analyzed for each experiment (A-D). Quartiles are assigned from median values (median values of total, upper 50% and lower 50% of the mean cell speed distribution) in A and B. 1Q = 1st Quartile. Student's t-test value: ****, p < 0.0001.