Skip to main content
Figure 3 | BMC Neuroscience

Figure 3

From: Comparison of slow and fast neocortical neuron migration using a new in vitromodel

Figure 3

Characterization of lattice cultures. A) Apposed cells are immunopositive for the neuronal marker doublecortin (Dcx) and a marker of excitatory neurons, VGLUT1 at E15 and E17. E17 cultures contained significantly more GABA expressing apposed cells and fewer nestin expressing apposed cells. B-F) Images from E17 cultures. G) Apposed cells in E15 and E17 cultures differ in the expression of layer specific cellular markers, CR50+ (layer 1), Cux-1+ (layer 2–4), Brn1+ (layer 2–5), FoxP2+ (layer 5–6). H-K) Images from E17 cultures. L) Nestin immunoreactivity of nodes and fiber fascicles in an E17 culture. M) DIC image of N) a Dcx+ (green) neuron apposed to a nestin+ fiber (red). Hoechst nuclear stain is blue. O) DIC image of an apposed cell P) α-tubulin immunolocalization reveals the characteristic tubulin cage (green) encasing the nucleus (blue). Q-T) Morphology of migrating neurons. Q) Leading process (LP) length at E17 in apposed cells transfected with eGFP expressing Sindbis virus; average length was 57.3 ± 6.7 μm (n = 9). R) Transmitted light and S) fluorescence images of apposed neurons (arrows). Inset is digitally magnified and reveals 3 filopodia at the end of the LP (arrowhead). T) Overlay. Scale bars: (F, K) 10 μm, (L) 500 μm, (P) 5 μm, (T) 20 μm. Immunocytochemical quantification was performed on a minimum of 200 cells from 3 separate cultures at E15 and E17. Analyses of fiber fascicles was performed on a minimum of 40 fascicles from 2 separate experiments at E15 and E17. Student's t-test values: *, p = 0.05; **, p = 0.01; ***, p < 0.001.

Back to article page