Figure 1

Effects of CBX and GZA on population-level bursting by the preBötC. A. Sample traces of extracellular activity recorded and integrated using a 50 ms time constant in the presence of GZA or CBX. Time bar represents 20 seconds. B. Whereas CBX decreased mean burst frequency within 20 min of application (i.), slices bathed in aCSF containing 50 μM GZA continued bursting at or near baseline frequency throughout recording (Two-way RM ANOVA with treatment by time as the source of variation d.f. = 4, F = 24.025, P < 0.001). B.ii. CBX decreased the area of integrated population bursts (Two-way RM ANOVA with treatment by time as the source of variation d.f. = 4, F = 25.783, P < 0.001). B.iii., Decreased burst area with CBX treatment appears to have been due to CBX decreasing burst amplitude beginning at 30 min of treatment (Two-way RM ANOVA with treatment by time as the source of variation d.f. = 4, F = 59.14, P < 0.001), as burst duration was seemingly unaffected by CBX up to the point at which bursting ceased (B.iv). *CBX value different from aCSF control at P < 0.05. **In these figures, the 60 min data point is presented to underscore that CBX terminated preBötC bursting within 1 h of treatment.