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Table 6 Relative transcription levels of METC components

From: Differential production of superoxide by neuronal mitochondria

Gene

Subunit

 

RGC-5

RGC-5 +SS

VDAC

-

ΔCt

-1.78

-1.73

  

Δ(ΔCt)

0.0

0.0

  

Relative Expression

1.0

1.0

SOD2

-

ΔCt

1.57

1.83

  

Δ(ΔCt)

3.35

3.67

  

Relative Expression

0.098

0.08

PMP70

-

ΔCt

1.30

1.60

  

Δ(ΔCt)

3.08

3.33

  

Relative Expression

0.118

0.099

C I

NDUFA9

ΔCt

0.88

0.67

  

Δ(ΔCt)

2.67

2.40

  

Relative Expression

0.157

0.189

C II

SDHA

ΔCt

4.49

3.33

  

Δ(ΔCt)

6.28

5.07

  

Relative Expression

0.013

0.030

C III

UQCRC2

ΔCt

3.80

2.92

  

Δ(ΔCt)

5.58

4.65

  

Relative Expression

0.021

0.039

C IV

MTCO1

ΔCt

9.58

7.38

  

Δ(ΔCt)

11.37

9.12

  

Relative Expression

0.00038

0.0018

C V

ATP5A1

ΔCt

0.48

0.98

  

Δ(ΔCt)

2.27

2.72

  

Relative Expression

0.208

0.152

  1. Changes in transcription levels of each gene studied are given in terms of the difference in threshold cycle compared to cerebral cells(ΔCt), the difference in threshold cycle relative to that of VDAC (Δ(ΔCt)), and the relative expression level, given as a percent of cerebral and normalized to VDAC expression.