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Table 4 Superoxide Production in Mitochondria from Differentiated and Undifferentiated RGC-5 Cells in the Presence of a Complex II Substrate and a Complex III Inhibitor.

From: Differential production of superoxide by neuronal mitochondria

 

Undifferentiated RGC-5

Differentiated RGC-5

 

N = 8

N = 2

 

Mean

SEM

Mean

SEM

Mitochondria

0.028

0.005

0.041

0.002

Succinate

0.031

0.005

0.037

0.001

Antimycin A

0.024

0.004

0.040

0.002

  1. The rates of superoxide production were determined after the addition of the complex II substrate succinate (10 mM) and the complex III inhibitor antimycin A (0.5 μM). Superoxide was indirectly measured using the H2O2 probe Amplex Red in the presence of horseradish peroxidase (HRP). The values are given in nmol H2O2/min/mg protein ± standard error of the mean (SEM), with n being the number of independent samples for each mitochondria type.