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Table 4 Superoxide Production in Mitochondria from Differentiated and Undifferentiated RGC-5 Cells in the Presence of a Complex II Substrate and a Complex III Inhibitor.

From: Differential production of superoxide by neuronal mitochondria

  Undifferentiated RGC-5 Differentiated RGC-5
  N = 8 N = 2
  Mean SEM Mean SEM
Mitochondria 0.028 0.005 0.041 0.002
Succinate 0.031 0.005 0.037 0.001
Antimycin A 0.024 0.004 0.040 0.002
  1. The rates of superoxide production were determined after the addition of the complex II substrate succinate (10 mM) and the complex III inhibitor antimycin A (0.5 μM). Superoxide was indirectly measured using the H2O2 probe Amplex Red in the presence of horseradish peroxidase (HRP). The values are given in nmol H2O2/min/mg protein ± standard error of the mean (SEM), with n being the number of independent samples for each mitochondria type.