| Undifferentiated RGC-5 | Differentiated RGC-5 |
---|
 | N = 8 | N = 2 |
---|
 |
Mean
|
SEM
|
Mean
|
SEM
|
---|
Mitochondria
| 0.028 | 0.005 | 0.041 | 0.002 |
Succinate
| 0.031 | 0.005 | 0.037 | 0.001 |
Antimycin A
| 0.024 | 0.004 | 0.040 | 0.002 |
- The rates of superoxide production were determined after the addition of the complex II substrate succinate (10 mM) and the complex III inhibitor antimycin A (0.5 μM). Superoxide was indirectly measured using the H2O2 probe Amplex Red in the presence of horseradish peroxidase (HRP). The values are given in nmol H2O2/min/mg protein ± standard error of the mean (SEM), with n being the number of independent samples for each mitochondria type.