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Table 3 Superoxide Production in Mitochondria from Differentiated and Undifferentiated RGC-5 Cells in the Presence of a Complex I Substrate and Inhibitor.

From: Differential production of superoxide by neuronal mitochondria

  Undifferentiated RGC-5 Differentiated RGC-5
  N = 8 N = 2
  Mean SEM Mean SEM
Mitochondria 0.030 0.004 0.033 0.004
Glutamate/Malate 0.046 0.004 0.052 0.003
Rotenone 0.048 0.004 0.055 0.003
  1. The rates of superoxide production were determined after the addition of the complex I substrates glutamate (10 mM) and malate (5 mM) and the complex I inhibitor rotenone (6.7 μM). Superoxide was indirectly measured using the H2O2 probe Amplex Red in the presence of horseradish peroxidase (HRP). The values are given in nmol H2O2/min/mg protein ± standard error of the mean (SEM), with n being the number of independent samples for each mitochondria type.