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Table 3 Superoxide Production in Mitochondria from Differentiated and Undifferentiated RGC-5 Cells in the Presence of a Complex I Substrate and Inhibitor.

From: Differential production of superoxide by neuronal mitochondria

 

Undifferentiated RGC-5

Differentiated RGC-5

 

N = 8

N = 2

 

Mean

SEM

Mean

SEM

Mitochondria

0.030

0.004

0.033

0.004

Glutamate/Malate

0.046

0.004

0.052

0.003

Rotenone

0.048

0.004

0.055

0.003

  1. The rates of superoxide production were determined after the addition of the complex I substrates glutamate (10 mM) and malate (5 mM) and the complex I inhibitor rotenone (6.7 μM). Superoxide was indirectly measured using the H2O2 probe Amplex Red in the presence of horseradish peroxidase (HRP). The values are given in nmol H2O2/min/mg protein ± standard error of the mean (SEM), with n being the number of independent samples for each mitochondria type.
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BMC Neuroscience

ISSN: 1471-2202

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