Quantitative Real-Time RT-PCR of Mitochondrial Components and Associated Proteins. Amplification curves for (A) VDAC, (B) Complex I, and (C) Complex IV. Solid black lines indicate undifferentiated RGC-5 cells, long dashes indicate differentiated RGC-5 cells, and short dashes indicate cerebral cells. The fuzzy line denotes the template-free control. The horizontal gray line indicates the calculated threshold value by which Ct is determined.