Figure 4
From: Differential production of superoxide by neuronal mitochondria
Superoxide Production in Cerebral, Neuroblastoma, and RGC-5 Mitochondria after Complex III inhibition. Mitochondria isolated from cerebral, neuroblastoma, and undifferentiated RGC-5 cells were normalized for protein content and superoxide production was analyzed after the addition of the complex II substrate succinate and the complex III inhibitor antimycin A. The mitochondrial superoxide production levels in cerebral cells were significantly higher than that of undifferentiated RGC-5 cells in the presence of succinate and after complex III inhibition with antimycin A. Both succinate and antimycin A elicited minimal superoxide production in undifferentiated RGC-5 cells, while in cerebral cells there was a near two-fold increase in superoxide production after the addition of antimycin A.