Table 1 Comparison of average microarray and qPCR results for 6 selected genes. 3 genes downregulated in Hdh-KO cells are shown in blue, 3 genes upregulated in Hdh-KO cells are shown in red. The average signals obatined in Microarray and qPCR experiments are shown for each gene. Also shown are primers used for qPCR experiments and the fold change calculated from microarry and qPCR data by dividing average Hdh-KO signal to average Hdh-HET signal.
Gene | GeneBank Acc # | Microrray | Q-PCR | |||||
|---|---|---|---|---|---|---|---|---|
HET | KO | Fold | Primers (FP/RP) | HET | KO | Fold | ||
Hdh | NM_010414 | 303.7 | -0.9 | DOWN | CGTGAGCATCTGCCAACATT CACACCGAGGATCAGGAGAGT | 404.3 | 2.0 | -202.2 |
Sox-2 | NM_011443 | 233.7 | -5.6 | DOWN | GATGCACAACTCGGAGATCAG GCTTCTCGGTCTCGGACAA | 16615.9 | 18.4 | -903.0 |
Tcf2 | NM_009330 | 255.6 | 7.3 | -35.0 | CACCTGACAGTAAAATGCAGATCA GAGTGTGTGCTGTGGATATTCGT | 4513.7 | 2.6 | -1736.0 |
Cart1 | NM_172553 | -9.3 | 286.9 | UP | CCCTGAGAACGGAGCTCACT TTGGCCCTTCGATTTTGAA | 12.5 | 45701.8 | 3656.1 |
Esm1 | NM_023612 | -35.6 | 498.6 | UP | GCGAGGAGGATGATTTTGGT GCATTCCATCCCGAAGGT | 19.7 | 2966.9 | 150.6 |
Pitx2 | NM_011098 | 124.8 | 1811.8 | 14.5 | CCGAGTCCGGGTTTGG TTGCGTTCCCGCTTTCTC | 21.3 | 368.2 | 17.3 |