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Table 1 List of proteins identified in synaptosomes and crude cytosol from hippocampal culture or tissue

From: Protein fingerprints of cultured CA3-CA1 hippocampal neurons: comparative analysis of the distribution of synaptosomal and cytosolic proteins

Spot number Accession number* Protein name S tis S cul C cul Method of detection
Cytoskeletal and their interacting Proteins(CK)
4 P60711 Actin, cytoplasmic x x x 2DE-MS
37 P63259 Actin, cytoplasmic 2 x x x 2DE-MS
48 Q61274 Alpha-cardiac actin [Fragment] x x   2DE-MS
100, 101, 123 P68370 Tubulin alpha-1 chain x x x 2DE-MS
36, 39, 47,124 P04691 Tubulin beta chain x x x 2DE-MS
22 P37805 Neuronal protein 25 x x   2DE-MS
53 P13668 Stathmin x x x 2DE-MS
119 P13668 Stathmin    x 2DE-MS
55 Q63228 Glia maturation factor beta x x x 2DE-MS
33 P45592 Cofilin, non-muscle isoform x x x 2DE-MS
92 Q9R0P5 Destrin x x x 2DE-MS
50 P63029 Translationally controlled tumor protein x    2DE-MS
125 P62963 Profilin I   x x 2DE-MS
127 Q9EPC6 Profilin II   x x 2DE-MS
Metabolism(M)
18, 105, 106 P04797 Glyceraldehyde-3-phosphate dehydrogenase x x x 2DE-MS
71 P16617 Phosphoglycerate kinase, testis specific x x x 2DE-MS
96 P10719 ATP synthase beta chain, mitochondrial x x x 2DE-MS
11 P31399 ATP synthase D chain, mitochondrial x x   2DE-MS
26, 27 P04764 Alpha enolase x x x 2DE-MS
84 P11980 Pyruvate kinase, M2 isozyme x x x 2DE-MS
78 Q9ER34 Mitochondrial aconitase [Precursor] x x x 2DE-MS
2, 67 P10860 Glutamate dehydrogenase, mitochondrial [Precursor] x x x 2DE-MS
34, 35, 64 P04636 Malate dehydrogenase, mitochondrial [Precursor] x x   2DE-MS
49 P11240 Cytochrome c oxidase polypeptide VA x x x 2DE-MS
8, 85 Q99NA5 NAD+specific isocitrate deihydrogenase a-subunit x x   2DE-MS
83 P42123 L-lactate dehydrogenase B chain x x x 2DE-MS
5 P48500 Triosephosphate isomerase x x x 2DE-MS
31, 103 P05065 Fructose-biphosphate Aldolase A x x x 2DE-MS
40 P09117 Fructose-bisphosphate aldolase C x x x 2DE-MS
41, 68 Q9D0K2 Succinil-CoA:3-ketoacid-coenzyme A transferase 1, mitochondrial x x x 2DE-MS
56 Q05982 Nucleoside diphosphate kinase A x x x 2DE-MS
61 P19804 Nucleoside diphosphate kinase B x x x 2DE-MS
69 P09606 Glutamine synthetase x x x 2DE-MS
70 P25809 Creatine kinase, ubiquitous mitochondrial [Precursor] x   x 2DE-MS
75 P13221 Aspartate aminotransferase cytoplasmatic x x x 2DE-MS
122 P07335 Creatine kinase, B chain x x x 2DE-MS
1, 82 O88989 Cytosolic malate dehydrogenase x x x 2DE-MS
80 P97532 3-mercaptopyruvate sulfurtransferase x    2DE-MS
90 P04764 Alpha-enolase x   x 2DE-MS
104 Q80YG1 Phosphoserine aminotransferase   x x 2DE-MS
113, 114 P48500 Triosephosphate isomerase   x x 2DE-MS
16 P19234 NADH-ubiquinone oxidoreductase 24 kDa subunit x x x 2DE-MS
Synaptic components (SY)
72, 76, 86, 87 P47942 Dihydropyrimidinase related protein-2 (drp-2) x x x 2DE-MS
89 Q62952 Dihydropyrimidinase related protein-3 (drp-3) x x x 2DE-MS
3 Q63537 Synapsin II x x x 2DE-MS
29 P07825 Synaptophysin x x   WB
30 P32851 Syntaxin 1 A-E x x   WB
46 P37377 Alpha-synuclein x x   2DE-MS
45 Q63754 Beta-synuclein x x   2DE-MS
73 P25286 Vacuolar proton translocating ATPase subunit a isoform 1 x x   2DE-MS
9, 51 P31044 Phosphatidylethanolamine-binding protein 1 x x x 2DE-MS
19 P55051 Fatty acid-binding protein, brain x x x 2DE-MS
126 P11030 Acyl-CoA-binding protein   x x 2DE-MS
128 P62775 Myotrophin    x 2DE-MS
Receptors, Ion Channel and adhesion molecules (I)
23, 63 Q6P9W9 Voltage-dependent anion-selective channel protein 1 x x x 2DE-MS
66 P50516 Vacuolar ATPsynthase catalytic subA x x   2DE-MS
Vesicle Transport and Recycling (T)
97 P46462 Transitional endoplasmic reticulum ATPase x   x 2DE-MS
25 P63012 Ras-related protein rab-10 x x x WB
28 Q62824 Exocyst complex component Sec8   x   WB
Protein Fate (synthesis, folding, modification and destination) (PF)
38 P63039 60 kda heat shock protein, mitochondrial precursor (hsp60) x x x 2DE-MS
65 P63017 Heat shock 70 kDa protein-ps1 x x x 2DE-MS
102 P07901 Heat shock protein [Fragment]    x 2DE-MS
44 P48721 Stress-70 protein, mitochondrial [Precursor] x x x 2DE-MS
17 Q00981 Ubiquitin carboxyl-terminal hydrolase isozyme L1 x x   2DE-MS
15 P20108 Mitochondrial thioredoxin-dependent peroxide reductase x x x 2DE-MS
52 Q9DB15 39S ribosomal protein L12, mitochondrial x x   2DE-MS
6 P52555 Endoplasmic reticulum protein ERp29 x x x 2DE-MS
32, 98, 99 P10111 Peptidyl-prolyl cis-trans isomerase A x x x 2DE-MS
42, 43, 79 P11598 Protein disulfide isomerase A3 x x x 2DE-MS
77 O35814 Stress induced phosphoprotein 1 x x x 2DE-MS
88 P11983 T complex polypeptide 1b x x x 2DE-MS
21 P55053 Fatty acid-binding protein, epidermal x x x 2DE-MS
59 P07483 Fatty acid-binding protein, heart x x x 2DE-MS
57 Q9EQX9 Ubiquitin-conjugating enzyme e2 x x x 2DE-MS
108, 109 P04905 Glutathione S-transferase Yb-1    x 2DE-MS
107 P04904 Glutathione S-transferase Yc-1    x 2DE-MS
112 P08010 Glutathione S-transferase Yb-2    x 2DE-MS
111 P08009 Glutathione S-transferase Yb-3    x 2DE-MS
116 P14942 Glutathione S-transferase alpha-4    x 2DE-MS
110 Q63716 Peroxiredoxin-1   x x 2DE-MS
54 P35704 Peroxiredoxin-2 x x x 2DE-MS
93 Q9R063 Peroxiredoxin-5, mitochondrial [Precursor] x x x 2DE-MS
94 Q63347 26S protease regulatory subunit 7    x 2DE-MS
115 P17220 Proteasome subunit alpha type 2   x x 2DE-MS
91 Q9JHW0 Proteasome subunit beta type 7 [Precursor] x   x 2DE-MS
Signalling Proteins (S)
24 P10354 Chromogranin A x x   WB
74 P50399 RAB GDP dissociation inhibitor beta-2 x x x 2DE-MS
10 P60766 Cell division control protein 42 homolog x x   2DE-MS
60 P70349 Histidine triad nucleotide-binding protein x x x 2DE-MS
81 P14669 Annexin III x    2DE-MS
117 Q62658 FK506-binding protein 1°   x x 2DE-MS
120 P62260 14-3-3 protein epsilon   x x 2DE-MS
121 P63102 14-3-3 protein zeta/delta   x x 2DE-MS
62 P62994 Growth factor receptor bound protein 2 x x   2DE-MS
Other (O)
12 O35264 Platelet-activating factor acetylhydrolase IB subunit beta x   x 2DE-MS
13 P67779 Prohibitin x x x 2DE-MS
7 O35244 Antioxidant protein 2 x x x 2DE-MS
20 P07632 Superoxide dismutase [Cu-Zn] x x x 2DE-MS
58 Q9CPT4 IL25 x x x 2DE-MS
14 O88767 CAP1 PROTEIN x x x 2DE-MS
118 P01041 Cystatin B    x 2DE-MS
95 Q8CDL9 Similar to hypothetical protein x x x 2DE-MS
  1. S: synaptosome; C: crude cytosol; cul: culture; tis: tissue; 2DE-MS: two-dimensional electrophoresis and MALDI-TOF mass spectrometry; WB: Western blot; * Swiss-Prot database; Spot numbers correspond to the spot numbers indicated in Fig. 1 and 4.