TY - JOUR AU - Hau, Susann AU - Reich, Doreen M. AU - Scholz, Markus AU - Naumann, Wilfried AU - Emmrich, Frank AU - Kamprad, Manja AU - Boltze, Johannes PY - 2008 DA - 2008/02/29 TI - Evidence for neuroprotective properties of human umbilical cord blood cells after neuronal hypoxia in vitro JO - BMC Neuroscience SP - 30 VL - 9 IS - 1 AB - One of the most promising options for treatment of stroke using adult stem cells are human umbilical cord blood (HUCB) cells that were already approved for therapeutic efficacy in vivo. However, complexity of animal models has thus far limited the understanding of beneficial cellular mechanisms. To address the influence of HUCB cells on neuronal tissue after stroke we established and employed a human in vitro model of neuronal hypoxia using fully differentiated vulnerable SH-SY5Y cells. These cells were incubated under an oxygen-reduced atmosphere (O2< 1%) for 48 hours. Subsequently, HUCB mononuclear cells (MNC) were added to post-hypoxic neuronal cultures. These cultures were characterized regarding to the development of apoptosis and necrosis over three days. Based on this we investigated the therapeutic influence of HUCB MNC on the progression of apoptotic cell death. The impact of HUCB cells and hypoxia on secretion of neuroprotective and inflammatory cytokines, chemokines and expression of adhesion molecules was proved. SN - 1471-2202 UR - https://doi.org/10.1186/1471-2202-9-30 DO - 10.1186/1471-2202-9-30 ID - Hau2008 ER -