Immunostaining of HDC and peripherin. We preformed double immunocytochemistry for HDC and peripherin. There is an extensive colocalization of the two proteins in the TM neurons. In A, the ventral/lateral part of the TM nucleus is stained for HDC in red. In B the same area is stained for peripherin (green). The staining for the two proteins is digitally merged in C. Neurons that are stained for both HDC and peripherin appear yellow in this picture. Most neurons are stained for both antigens. Scale bar 20 μm.