Figure 5

SC-like cells can induce the differentiation of SH-SY5Y neuroblastoma cells. SH-SY5Y cells were cultured in DMEM plus 2% FBS (control group) or SC-like cell-CM for 3 days. A, B: Under phase contrast, neurite outgrowth is minimal-to-no in control group (A), whereas many of the SH-SY5Y cells extend long neurites in SC-like cell-CM group (B). C and D: Immunocytochemistry of beta-tubulin III in control group and SC-like cell-CM group. Few or no SH-SY5Y cells are beta-tubulin III positive in control group (C), whereas 31 ± 6% of the SH-SY5Y cells are beta-tubulin III positive in SC-like cell-CM group (D). Nuclei are labeled with DAPI (blue). Bar, 50 μm.