Figure 8

Immunocytochemical characteristics of brain tumor mass from SCID mouse transplanted with radio-resistant cancer stem cells derived from human gliobalstoma cells. Frozen sections of brain tumors were labeled with antibodies to human-nuclei to recognize engrafted human gliobalstoma cells (Red) or von Willebrand factor to recognize blood vessels (Green). Fluorescence microscope images of solid tumors from brain tissue transplanted with radio-resistant A172 (C-F) and GBM2 (G-J) cells. (A-B) Demonstrates a human-nuclei (red) and von Willebrand factor of solid tumors as the result of transplantation with radio-resistant cells (× 100 magnification). The sections were also labeled with DAPI (blue) to identify nuclei. Laser scaning confocal images of blood vascular tissue from brain samples by transplantation with radio-resistant A172 (C-F) and GBM2 (G-J) cells (× 100 magnification). (C-J) Demonstrates a von Willebrand factor (green), human-nuclei (red) of blood vascular, and TOPRO-3 (blue) derived from radio-resistant A172 (C-F) and GBM2 (G-J) cells (× 100 magnification). G and K are merged Confocal images derived from radio-resistant A172 (C) and GBM2 (G) cells, respectively. Scale bar, 50 μm.