Figure 6

In vitro differentiation and cell surface marker coexpression of radio-resistant cells derived from human glioblastoma cells. Cells were labeled with antibodies for neuronal (Tuj and MAP2ab; red), glial (GFAP; red), and neural stem cells (Nestin; red), and cell surface epitopes (CD133, CD117, and CD45; green), and were then observed via laser scanning confocal microscopy. (A) Laser scanning images of differentiation and CD133 cell surface marker coexpression of radio-resistant human glioblastoma A172 and GBM2 cells. (B) Laser scanning images of differentiation and CD117 cell surface marker coexpression of radio-resistant human glioblastoma A172 and GBM2 cells. (C) Laser scanning images of differentiation and CD133 cell surface marker coexpression of radioresistant human glioblastoma A172 and GBM2 cells. (D) Laser scanning images of differentiation and CD71 cell surface marker coexpression of radioresistant human glioblastoma A172 and GBM2 cells. Images represent × 400 magnification. Scale bars, 25 μm.