Identification of PNNs with antibody against the ADAMTS-derived fragment of brevican. Mouse PNNs immunoreactive for the ADAMTS-derived fragment of brevican distinguished with anti-EAMESE (green) (A) were broadly distributed in cerebral cortex, but were especially prominent deep in cortical layer IV, which differs from the distinct pattern of WFA staining of PNNs (B). The most intense region of WFA reactivity appears in cortical layer III of primary somatosensory cortex (B-C). Scattered WFA positive PNNs are also found in layer V. Images A-C were captured at 100× magnification. Scale bar represents 100 μm.