Chondroitinase treatment of brain sections prior to binding by WFA. Binding of Wisteria floribunda agglutinin (WFA) lectin after treatment with Chase. Paraformaldehyde-fixed coronal sections from (A and C) rat and (B and D) mouse brain were probed with biotinylated-WFA (A and B) before and (C and D) after Chase treatment. Arrow = retrosplenial cortex; arrow head = parietal cortex. Note the near elimination of reactivity after Chase treatment. All images were captured at 25× magnification. Scale bar represents 100 μm.