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Figure 2 | BMC Neuroscience

Figure 2

From: Discordant localization of WFA reactivity and brevican/ADAMTS-derived fragment in rodent brain

Figure 2

N-terminal and neoepitope fragment formed by ADAMTS cleavage of brevican. Recombinant ADAMTS4 cleavage product of rat brevican is recognized by anti-EAVESE: (A) Proteoglycan-containing fraction eluates of rat brain extracts from a DEAE cation exchange matrix were probed with anti-brevican. (B) Proteoglycan fraction was probed with anti-EAVESE, antibody raised against the C-terminal neoepitope sequence of the ADAMTS-cleaved N-terminal fragment of brevican. (C) Proteoglycan fraction after incubation with recombinant ADAMTS4 and probed with anti-brevican or (D) anti-EAVESE. After incubation with human recombinant ADAMTS4, brevican was proteolytically cleaved resulting in diminished full length brevican and the appearance of a 55 kD N-terminal fragment recognized by both anti-brevican and anti-EAVESE.

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