Infection of neuronal cells followed by staurosporine incubation. SK-N-MC neuroblastoma cells infected with Chlamydia pneumoniae for 3 days (A) and 10 days (B), revealing chlamydial inclusions (green). In panel A, Imagen anti-chlamydial specific antibody was used. In panel B, anti-chlamydial antibodies 60C19 and Imagen were used in combination to detect prolonged or persistent infection. Nuclei were identified by Hoechst stain (blue), and the cytoplasm was stained with Evans blue (red). Mag. bar = 10 μm.