Figure 9
Antiphase cycling of PER expression in DN 2 s from L1 larvae. CNSs were dissected from wild-type (W.T.) L1 larvae collected at ZT21 and ZT9, immunostained with CLK and PER antibodies, and imaged by confocal microscopy. Dorsal is at the top. (A-C) A 21 μm projected Z-series image of CLK (A), PER (B), and merged CLK + PER (C) IR in W.T. L1 larvae collected at ZT21. CLK IR is present in DN1, DN2 and s-LNv cells (A, C) and PER IR is detected in DN1 and s-LNv cells (B, C). (D-F) A 22 μm projected Z-series image of CLK (D), PER (E), and merged CLK + PER (F) IR in W.T. L1 larvae collected at ZT9. CLK is present in DN1, DN2 and s-LNv cells (D, F) and PER IR is detected in DN2 cells (E, F). Co-localization of CLK (red) and PER (green) is shown in yellow. All images are representative of three or more independent experiments.