Figure 8

CLK and PER expression in embryos during ES 16 and ES 17. 0 h – 24 h wild-type (W.T.) embryos were collected at CT33, immunostained with CLK and PER antisera, and imaged by confocal microscopy. Anterior is to the left and dorsal is on the top. (A-C) 58 μm Z-series projection of PER (A), CLK (B) or CLK + PER (C) IR in the brain (box) and VNC during early ES 16. (A'-C') Magnified view of the boxed brain regions in panels A-C, respectively. (D-F) 18 μm Z-series projection of PER (D), CLK (E) or CLK + PER (F) IR in the brain (box) and VNC during late ES 16. (D'-F') Magnified view of the boxed brain regions in panels D-F, respectively. (G-I) 20 μm Z-series projection of PER (G), CLK (H) or CLK + PER (I) IR in the brain (box) and VNC during ES 17. (G'-I') Magnified view of the boxed brain regions in panels G-I, respectively. Co-localization of CLK (red) and PER (green) is shown as yellow. All images are representative of three or more independent experiments.