Figure 7

PER and CLK expression in ES 12 – ES 15 embryos. 0 h – 24 h wild-type (W.T.), per⁰¹, or Clk^Jrk embryos were collected at CT33, immunostained with CLK and PER antisera, and imaged by confocal microscopy. (A, B) 12 μm Z-series projection of PER (A) or CLK + PER (B) IR in the VNC of a W.T. embryo during ES 12. (C, D) 20 μm Z-series projection of PER (C) or CLK + PER (D) IR in the VNC of a W.T. embryo during ES 13. (E, F) 18 μm Z-series projection of PER (E) or CLK + PER (F) IR in the brain (box) and VNC of a W.T. embryo during ES 14. (G, H) 30 μm Z-series projection of PER (G) or CLK + PER (H) IR in the brain (box) and VNC of a W.T. embryo during ES 15. (I) 42 μm Z-series projection of PER (E) or CLK + PER (F) IR of a per⁰¹ embryo during ES 15. (J) 22 μm Z-series projection of PER (E) or CLK + PER (F) IR in the brain (box) and VNC of a Clk^Jrk embryo during ES 15. Co-localization of CLK (red) and PER (green) is shown as yellow. All images are representative of three or more independent experiments.