Truncated CLKJrk protein accumulates to low levels. (A) Western blot containing 100 μg of head extract from ClkJrk or wild-type flies collected at ZT1 and probed with CLK GP50 antiserum. Full length CLK and truncated CLKJrk proteins are denoted in short (left) or long (right) exposures of the blot. (B) Brains from ClkJrk L3 larvae were dissected and fixed at ZT1, immunostained with a lower dilution of CLK GP50 antibody (diluted 1:1000 instead of 1:3000), and imaged by confocal microscopy. Images show a 28 μm Z-series projection of a right brain hemisphere, where dorsal is at the top. CLK GP50 immunostaining is detected in DN1*, DN2 and LN cells. DN1* and DN2 cells are as defined in the legend for Fig. 2, and LN cells include s-LNvs, l-LNvs and LNds. All images are representative of three or more independent experiments.