Figure 6

LPA and S1P induce ERK phosphorylation in hES-NEP cells. hES-NEP cells were treated with 1 μM LPA (A) or 100 nM S1P (B) for the indicated amounts of time and then assayed for phosphorylated p44/42 ERK and total p44/42 ERK as described in Materials and Methods. In each case, results are shown as a Western blot image of a representative experiment and a densitometry graph of combined data from two independent experiments analyzed using an unpaired 2-tailed t-test *: p < 0.05; **: p < 0.01; ***: p < 0.001.