hES-NEP cells express functional G
-coupled LPA and S1P receptors. (A) hES-NEP cells were treated with 50 μM forskolin and 10 μM of each of the indicated drugs for 20', then assayed for cAMP levels as described in Materials and Methods. hES-NEP cells were treated with 50 μM forskolin and various concentrations of LPA (B) or S1P (C) for 20' in the presence (star) or absence (square) of Ptx. Data are consistent with three independent experiments.