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Table 1 Antisera used to characterize TrpM5 cells

From: Is TrpM5 a reliable marker for chemosensory cells? Multiple types of microvillous cells in the main olfactory epithelium of mice

Antisera against

Marker for

Company

Lot

Calbindin

calcium-binding protein involved in calcium signaling

SWANT CB38

9.03

Calretinin

calcium-binding protein involved in calcium signaling

SWANT 7699/4

18299

CGRP

trigeminal nerve fibers

Peninsula Lab. T-4032

040826-4

Chromogranin A

neuroendocrine cells

Santa Cruz Sc-1488

D0507

CK18

supporting cells

Chemicon MAB3234

0507004430

Espin

actin-binding protein in microvilli

Dr. J. Bartles, Northwestern University, Chicago

-

GFP

green fluorescent protein

Abcam AB290

207431

Galphaq/11

G-protein subunit q/11/14 – transduction component

Santa Cruz Sc-392

F1107

IP3R3

IP3 receptor 3 – transduction component

Chemicon AB9076

25041643

Na+, K+-ATPase

sodium-potassium pump

Biogenesis 0126-2000

773/272892

NSE

neuronal and neuroendocrine cells

DAKO M0878

05437

PDE2A

transduction component

Santa Cruz Sc-17227

L0402

PGP 9.5

neuronal and neuroendocrine cells

AbD Serotec 7663-0504

071207

OMP

olfactory neurons

Dr. F. Margolis, University of Maryland

-

P2X2

purinergic receptor – transduction component

Alomone Labs APR003

AN-06

P2X3

purinergic receptor – transduction component

Chemicon AB5895

0602021455

PDE2A

transduction component

Santa Cruz Sc-17227

L0402

PGP 9.5

neuronal and neuroendocrine cells

AbD Serotec 7663-0504

071207

PLCbeta2

transduction component

Santa Cruz Sc-206

B0907

SNAP 23

synapses

SynapticSystems 111202

-

SNAP 25

synapses

Calbiochem NE1014

30933

Substance P

trigeminal nerve fibers

Accurate YMC1021

E9381

SUS-1

supporting cells in the main olfactory epithelium

gift of Dr. F. Margolis, University of Maryland

-

SV2

synapses and some nerve fibers

Developmental Hybridoma Bank

-

Synaptophysin

synapses

Epitomics 1485-1

YE269

Substance P

trigeminal nerve fibers

Accurate YMC1021

E9381

TrpC6

transient receptor potential channel C6 – transduction component

Abcam AB12249

342108

VAChT

"membrane transport protein"

Chemicon AB1578

24080681

Villin

actin-binding protein in microvilli

Beckman-Coulter 0258

1D2C3